Clobenzorex (ASENLIX) is a drug appetite suppressant metabolized to amphetamine by the body, which causes difficulties in the interpretation of drug tests positive amphetamine. Previous studies have shown that the parent and metabolites excreted more urinary medicine. Clobenzorex itself was as long as 29 h detects administration, a detection limit of 1 ng / ml is used. Despite this fact, a plurality of samples of amphetamine-positive (> or = 500 ng / ml) did not contain any detectable clobenzorex. Thus, the absence clobenzorex not need to exclude the possibility of using urine. Finally, more considered use, evaluation of other metabolites assess the possibility of clobenzorex. One study reported the presence of unidentified hydroxy metabolites Clobenzorex as long as amphetamine was detected in some subjects. The viability of the use of a hydroxy metabolite evaluate the use of Clobenzorex in samples containing confirm amphetamine, 4-hydroxyclobenzorex was synthesized for this study. This metabolite detected easily be identified and has not been usually at a higher level than amphetamine in positive amphetamine urine, was recognized long after Clobenzorex itself. Samples from a study controlled single dose administered Clobenzorex obtained (30 mg) are involved, were analyzed for the presence of the 4-hydroxy metabolite. The analytical method used acid hydrolysis followed by extraction and liquid-liquid analysis by gas chromatography ion mass m / z Monitor 125, 330 and 364. 4-Hydroxyclobenzorex and 3-Cl were regioisomer used to identify and quantify the metabolites. 30-5: 00 Administration and distance of about 5705-88410 ng / ml concentrations up to 4-hydroxyclobenzorex found about 1 micron. More importantly, all samples, amphetamine contained in> or = 500 ng / mL also contained detectable amounts of the hydroxy metabolites (LOD 10 ng / ml), it is a valuable tool, use of illegal amphetamines Clobenzorex the differentiation.
