What is Acridine Orange

Product name: Acridine Orange
CAS: 10127-02-3
Molecular Formula: C34H40Cl4N6Zn
Molecular Weight: 739.9426
Product Description: orange powder. Soluble in water, ethanol. The solution for the orange fluorescent green belt. PH value of 8.4-10.4, no fluorescence. Can be between two adjacent pairs of bases, the part liberated from the double helix of DNA, the two bases of expansion of the spacer. Such a DNA replication process can increase the DNA strand or a missing base, which causes mutations in the reading frame. As a fluorescent tracer, tumor cells and bacteria, nucleic acid staining and mutagenic mutations of the reading frame. The acridine orange is a kind of fluorescent pigment, acridine orange filter wavelength of 488 nm. Filter wavelength blocking at 515 nm. Acridine orange combined with DNA and RNA in the volume of the cell, there is a difference, but a different color fluorescent specificity (grayed), this is because DNA is a high-absorption polymer The location of fluorescent material is lower, green fluorescence hair low degree of polymerization and RNA material can and fluorescent associated with the location of the greatest number, so that the red fluorescence of the hair.