The drugs encapsulated into exosomes was divided into a few steps?

A: The three steps.
First, find a somatic cell donor exosomes;
The second step, to find that the drug;
The third step, put it inside.
Exosomes potential as a drug delivery system (drug delivery system, DDS) targeting tool has already been found, and DDS-based exosomes have to look forward to the results oh.
what? Exosomes not make waves all day Hing tumors for cancer as reckless it?
Exosomes: In the past I did not have a choice, and now I want to be a good exosomes.
Following entered, we look at how the "medicine" put exosomes.
1. When the "drug" is when microRNA see an interesting experiment Y.Akao et.al of:
The first step: the peripheral blood lymphocytes isolated
Step two: differentiation, macrophages adherent
The third step: the way to the target RNA molecule (in this experiment, as miR-143BPs) transfected with liposome transfected into monocytes / macrophages, this process takes 12-16 hours
Step Four: After removing the supernatant 24 hours incubation in serum-free medium (this time the cells begin to secrete carry miR-143BPs exosomes a)
Step five: being elected MV / miR-143BPs and / or cell transfected transmitted back into the patient
In this experiment, after being transfected intravenous serum, cancer, kidney miR-143 levels in tumor cells grown model exosomes significantly increased.
2. When the "drug" is when siRNA, L Alvarez-Erviti et.al in nature biotechnology articles:
The first step: collection of mouse bone marrow (hematopoietic progenitor cells, osteoblasts, etc.), to be stimulated with interleukin.
Step Two: Get dendritic cells, introducing plasmids encoding Lamp2b protein target.
The third step: the supernatant obtained by ultracentrifugation was purified exosomes (its surface capable of binding to the target receptor-specific ligand).
Step four: the electroporation technique siRNA transfection exosomes
Step 5: eccrine new weight injected back into mice.
The experiment, siRNA was delivered to the brain cells and closed with Alzheimer's disease-related gene BACE1, the results showed that BACE1 gene activity is reduced by 60%.
3. Other drugs, because many species, but the methods are similar, but here is an example of a SuChul Jang et.al. Doxorubicin (doxorubicin) related experiments:
The first step: Get U937 (human monocytes) Raw264.7 (mouse macrophage), or incubated in RPMI medium containing 10% exosome-depleted FBS in 24 hours
Step two: density gradient ultracentrifugation, purified to obtain exosomes
The third step: Add Doxorubicin and other drugs, 37 ℃ incubated for 2 hours.
Step Four: The exosomes injected back into the living body.
The experiments show that EVs will CT26 colon adenocarcinoma cell enrichment, but will also enriched in the liver

Germany Xenios company i-cor synchronous heart assist system is eligible for CE mark

Xenios German medical technology companies in Europe, sales of i-cor synchronous heart assist system received CE mark, i-cor system is the world's first for cardiogenic shock and high-risk interventions heartbeat synchronization heart assist system that utilizes a small of the pump to provide assistance physiological heart beat. The system can use to help sync pulse were weak heart beat, also combined with myocardial protection measures, sync pulses can cover the entire patient weak heart.
Xenios said the new system compared to traditional methods can improve a patient's heart coronary blood flow, and can limit the cardiac afterload; Executive Director of the Company Georg Matheis representation, i-cor synchronous heart assist system has been approved by the European regulatory authorities will bring a new era through development assistance in the field of percutaneous heart; we will provide each heartbeat weakened heart artificial heart, had no such direct cardiac assist method.
Now this synchronization heart assist system, unlike transdermal continuous flow system, i-cor synchronous heart assist system will be the world's first synchronized for debilitating cardiac mechanical circulatory support systems; i-cor system not only for being cardiogenic shock patients suffer provide new treatment, cardiac catheterization laboratory can also help patients at high risk of intervention; heart assist system synchronization pulse physiological overlaid or superimposed pulse attenuation patient, compared to standard therapy and is also a minimally invasive treatment.

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How to buy galacturonic acid, galacturonic acid Sale

Product name:Polygalacturonic acid
CAS:25990-10-7
Molecular Formula:C6H10O7
Molecular Weight: 194.1394
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CAS: 7400-08-0
Molecular Formula: C9H8O3
Molecular Weight: 164.158
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