The drugs encapsulated into exosomes was divided into a few steps?

A: The three steps.
First, find a somatic cell donor exosomes;
The second step, to find that the drug;
The third step, put it inside.
Exosomes potential as a drug delivery system (drug delivery system, DDS) targeting tool has already been found, and DDS-based exosomes have to look forward to the results oh.
what? Exosomes not make waves all day Hing tumors for cancer as reckless it?
Exosomes: In the past I did not have a choice, and now I want to be a good exosomes.
Following entered, we look at how the "medicine" put exosomes.
1. When the "drug" is when microRNA see an interesting experiment Y.Akao et.al of:
The first step: the peripheral blood lymphocytes isolated
Step two: differentiation, macrophages adherent
The third step: the way to the target RNA molecule (in this experiment, as miR-143BPs) transfected with liposome transfected into monocytes / macrophages, this process takes 12-16 hours
Step Four: After removing the supernatant 24 hours incubation in serum-free medium (this time the cells begin to secrete carry miR-143BPs exosomes a)
Step five: being elected MV / miR-143BPs and / or cell transfected transmitted back into the patient
In this experiment, after being transfected intravenous serum, cancer, kidney miR-143 levels in tumor cells grown model exosomes significantly increased.
2. When the "drug" is when siRNA, L Alvarez-Erviti et.al in nature biotechnology articles:
The first step: collection of mouse bone marrow (hematopoietic progenitor cells, osteoblasts, etc.), to be stimulated with interleukin.
Step Two: Get dendritic cells, introducing plasmids encoding Lamp2b protein target.
The third step: the supernatant obtained by ultracentrifugation was purified exosomes (its surface capable of binding to the target receptor-specific ligand).
Step four: the electroporation technique siRNA transfection exosomes
Step 5: eccrine new weight injected back into mice.
The experiment, siRNA was delivered to the brain cells and closed with Alzheimer's disease-related gene BACE1, the results showed that BACE1 gene activity is reduced by 60%.
3. Other drugs, because many species, but the methods are similar, but here is an example of a SuChul Jang et.al. Doxorubicin (doxorubicin) related experiments:
The first step: Get U937 (human monocytes) Raw264.7 (mouse macrophage), or incubated in RPMI medium containing 10% exosome-depleted FBS in 24 hours
Step two: density gradient ultracentrifugation, purified to obtain exosomes
The third step: Add Doxorubicin and other drugs, 37 ℃ incubated for 2 hours.
Step Four: The exosomes injected back into the living body.
The experiments show that EVs will CT26 colon adenocarcinoma cell enrichment, but will also enriched in the liver