What is NCI N87

The aim of this study was to assess the human cell line NCI-N87 as a model for gastrointestinal permeability of drug trials in pH conditions of the stomach. Optimal conditions that NCI-N87 cells resulted in a typical differential stomach to form the epithelial barrier, have a seeding rate of 2.5 x 10⁵ cells / cm² on porous and deposits growth in RPMI-1640 medium supplemented serum in 18- 27 days after confluence. The resulting cell monolayers have electrical resistance (TEER) moderately high values ​​of transepithelial of about 500 Ω cm, the cells expressing the polygonal morphology E-cadherin and ZO-1 proteins on their contact surfaces, and mucus production clusters . Monolayers resisted set to 3.0 with the pH 7.4 apical base pH 7.4. The apparent permeability coefficient (P (APP)) model compounds were evaluated in the apical to basolateral and apical to basolateral direction under different pH gradient. The monolayers were impervious to the yellow marker lucifer integrity (low P (app) from 0.3 to 1.1 × 10⁻⁶ cm / s). Furosemide P (APP) (0.4-1.5 × 10⁻⁵ cm / s) were slightly pH dependent, but remained moderate. Caffeine P (app) (4.2 5.0 x 10⁻⁵ cm / s) were higher and insensitive to pH variations. The cell line NCI-N87 provides a useful tool in gastrointestinal medicine in vitro to evaluate the permeability and absorption in the physiological conditions in the human stomach.

1H-Tetrazolyl-1-acetic acid

Product Name:1H-Tetrazolyl-1-acetic acid

CAS:21732-17-2

Molecular Formula:C3H3N4O2

Molecular Weight:127.0821

Description:Tetrazol-1-acetic acid; Tetrazol-1-ylacetic acid; 1H-Tetrazolyl-1-Acetic Acid; Tetrazolyl Acetic Acid; 1H-tetrazol-1-ylacetato(3-); 1H-tetrazol-1-ylacetate

Transfluthrin price

Product name:Transfluthrin
CAS:118712-89-3
Molecular Formula:     C15H12Cl2F4O2
Formula Weight:371.16
Description:
Transfluthrin is a fast-acting pyrethroid insecticide with low persistence. It has the molecular formula C15H12Cl2F4O2. Transfluthrin can be used inside against flies, mosquitoes and cockroaches. It is a relatively volatile material and acts as a contact and inhalants.

What is HeLa S3

In contrast to previous reports, this study of HeLa, HeLa S3 cells, and Hep-2 revealed that karyotypes of each cell line are characterized by a composition of uniform chromosomal marker and a constant number of copies for both normal chromosomes and markers. Based on these fingerprints and chromosomal analysis of metaphases 50 modal karyotype is defined each cell line. Each modal karyotype was the composite of the content previously reported karyotypes of the same cell line, and in general, the former is the same or a higher number of chromosome copies that he had. These modal karyotype can be used as a standard to identify and further individualize both the cell line and a subline in this cell line. We also found that many cells of each cell line have the same karyotype. Digital data parts are compiled in a table format by which the amount of difference between chromosomes crops can be easily compared. Also coming soon are chromosomal changes discussed characteristics can help distinguish clonal derivatives derived from cross-contamination of cell lines lines.

MKN-45 for sale

Product name:MKN-45Species:Human
Source:Gastric
Specifications: Strains
Morphology:Both spindle-shaped or oval cells growing in monolayers and single round cells or clumps in suspension
Application:For research purposes
Medium:
80% RPMI 1640 + 20% FBS
Storage: Frozen with 70% medium, 20% FBS, 10% DMSO at about 1-3 x 106 cells/ampoule
Product description:
This cell line was established by Akiyama, S., derived from a 35-year-old suffers from signet ring cell carcinoma of the gastric lymph nodes of women.

Human Umbilical Vein Endothelial Cells

Product name:HUVEC(Human Umbilical Vein Endothelial Cells)
Specifications:5 x 105 cells, 1 ml

Type:Umbilical Cord Cell System

Product description:

HUVEC were isolated from human umbilical vein. HUVEC are cryopreserved at passage and provided frozen.HUVEC by the immunofluorescence method are with antibodies to vWF / Factor VIII and CD31 (P-CAM) and by absorption of Dil-Ac-LDL. HUVEC were negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeasts and fungi.

Alpha-fetoprotein summary

This gene encodes alpha-fetoprotein, a major plasma protein of the yolk sac and liver during fetal life generated. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistence of alpha-fetoprotein can also be found in individuals without any obvious disease. The protein is considered homologous albumin fetal serum, and alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is in the monomer as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in the amniotic fluid to be measured against the protein to detect spina bifida and anencephaly renal loss.