What is Rhizoma Corydalis Extract

Synergistic analysis of anti-cancer agents is an important method for determining the ratio and / or dose of drug for clinical therapy. However, this method is rarely used to assess the formulation composition of traditional Chinese medicine. "Yanhusuo San" (YHSS), which consists of Yanhusuo Rhizoma Corydalis and Ezhu (Rhizoma Curcumae), since the Song dynasty (960-1279). An archaic prescription of Chinese medicine. We have previously shown that one or Yanhusuo Ezhu possesses strong anti-cancer activity. We have tried to determine the possible synergistic effect between these two Chinese herbs. We measure the IC50 of each herbal extract and the two extracts in various dose ratios per MTT assay. Isobologram and combination index (IC) of the analysis were used to evaluate the synergistic effect of yanhusuo and Ezhu in various fixed ratios. Our results showed that the association of two herbal extracts the strongest effect against cancer cell proliferation in a ratio of 3: 2 (to Ezhu yanhusuo designated as E3Y2) a. With Boyden's chamber assay, flow cytometry and fluorescence microscopy analysis, it was found that E3Y2 could reduce cell invasion capacity and significantly induce cytochrome release instead of using only one time. However, E3Y2 could not affect the distribution of the cell cycle. If the ERK1 / 2, p-ERK1 / 2 and p-Rb values ​​were determined by Western blot analysis, we found that E3Y2 significantly suppressed P-ERK levels. Thus, our studies provide a plausible molecular basis for the synergistic anti-tumor effect of Ezhu and Yanhusuo.