SHG-44 glioma cell-line

GOAL:
To study the effect of tamoxifen on voltage-sensitive sodium channels in SHG-44 glioma cells.
METHODS:
To record the Na currents in the SHG-44 cell line, a whole patch clamp of the cell was used and studied the effect of tamoxifen with different concentrations on this channel flow.
RESULTS:
This channel has been activated and deactivated quickly. Tamoxifen was able to greatly reduce the amplitude of the Na currents of the SHG-44 cell line. This block effect is dose-dependent and voltage dependent. When the holding potential was 0 mV, 8 micromoles / L tamoxifen was 69% block these fluxes. The half inhibitory concentration (IC 50) is 5.54 micromoles / liter.
CONCLUSION:
Tamoxifen could significantly block the voltage-dependent sodium channel in the SHG-44e malignant glioma cell line. It could be one of the mechanisms that inhibit tamoxifen glioma proliferation. Clamp technique was used to record Na currents in SHG-44 cell line and study the effect of tamoxifen with different concentrations on this channel flow.
RESULTS:
This channel has been activated and deactivated quickly. Tamoxifen was able to greatly reduce the amplitude of the Na currents of the SHG-44 cell line. This block effect is dose-dependent and voltage dependent. When the holding potential was 0 mV, 8 micromoles / L tamoxifen was 69% block these fluxes. The half inhibitory concentration (IC 50) is 5.54 micromoles / liter.
CONCLUSION:
Tamoxifen could significantly block the voltage-dependent sodium channel in the SHG-44e malignant glioma cell line. It could be one of the mechanisms that inhibit tamoxifen glioma proliferation.

Hugh-Leifson Medium

For the first time the method of (1 hour) rapid screening was developed by groups of Gram-negative bacteria through the OF test with glucose using mikrovolumetrischen techniques. The method is based on the use of hydrogen peroxide at a non-bactericidal concentration as a constituent of a liquid medium containing a buffer and a glucose indicator and is provided for the oxidation of glucose. Introduced for the investigation of the bacterial environment catalase ensures rapid saturation of the medium with oxygen and the completion of glucose oxidation in 10-60 minutes. A similar period is necessary in order to obtain a complete fermentation of the glucose in the same medium without hydrogen peroxide. It has been demonstrated that significant results have been obtained in the joint study of the OF-tests on the 502 strains belonging to the 21 genera and the bacteria which ferment Gram-negative nichtfermentierender, according to the process of hydrogen peroxide and the medium of Hugh-Leifson.

L-Arginine L-Aspartate

1. Some of the effects of ouabain on the absorption of 14C labeled amino acids and 32P labeled orthophosphate in squid axons have been studied. 2. ouabain in artificial seawater reduced intake of glycine, L-alanine, L-arginine, L-aspartate and L-glutamate. After a period of 3 hours in the inhibition of ouabain and glycine L-glutamate absorption was marked. 3. After 3 hours in the axoplasmic ouabain Na concentration had increased by only about 14% and the K concentration decreased only by about 9%. It is therefore unlikely that changes in Na and K gradient across the membrane due to ouabain are the reason for inhibition of amino acid uptake. This was confirmed when the axons to carry out pulses at 200 / sec for 40 minutes were made. There was no subsequent inhibition of glycine and L-glutamate absorption, although the axoplasmic Na increased by about 42% and K decreased by about 37%. 4. A detailed examination of the time course of inhibition of amino acid uptake by ouabain, were used in the intracellular Szintillatortechniken showed that there was no inhibition for 30-100 Min, then it was before fairly quickly. This operation was not affected by the cycloheximide protein synthesis inhibitor. It is unlikely that the inhibition of the orthophosphate receiving a similar delay is subjected, as has reached a maximum after 40 min. 5. It was concluded that the inhibitory effects of ouabain on amino acid uptake in squid axons are not due to effects on the sodium and potassium gradients caused by inhibition of the Na-K pump . The retardation of inhibition may reflect the operation of an intracellular regulation mechanism, which is triggered when ouabain inhibits the pump, but the time required for operation. If the formation of a second inhibitory messenger molecule is involved, it is unlikely that it is a protein.

Morphine Hydrochloride for sale

Product Name: Morphine Hydrochloride
CAS: 52-26-6
Molecular formula: C17H19NO3 · HCl · 3H2O
Molecular Weight: 375.85
Product Description: This product is a potent analgesic that is for another pain reliever, acute pain, such as severe trauma, sores, burns, such as advanced cancer pain, applicable. Myocardial infarction (mid) and blood pressure is normal, use this product, calm patient and reduce the financial burden on the heart. Applied to cardiac asthma pulmonary edema can be temporarily attenuated. Patient before anesthesia and surgery in medicine can still penetrate into drowsiness. Because this product to smooth out muscle stimulation is stronger, so it can not for visceral colic (such as bravery, kidney colic, etc.) separate but should effectively spasmolysis medicine Share with atropine, etc. According to the World Organization for Health and Pain Treatment by the National Drug Administration under presented the three-step pattern, morphine is a representative drug for the treatment of severe pain.

Docetaxel Trihydrate price

Product Name: Docetaxel Trihydrate

CAS: 148408-66-6

Molecular weight: 861.94 g / mol

Molecular formula: C43H53NO14 · 3H2O

Description: It is a white to off-white crystalline powder. This product is applicable to the treatment of locally advanced or metastatic, locally advanced or non-small cell carcinoma of the metastatic lung even after failure of cisplatin-based chemotherapy.

How to buy Dextromethorphan

Product Name: dextromethorphan
CAS: 125-71-3
Molecular formula: C18H25NO
Molecular Weight: 271.4 g / mol
Detailed Description: Dextromethorphan is an antitussive (cough suppressant) drug. It is one of the active ingredients in many over-the-counter cold and cough medicines, such as Robitussin, NyQuil, Dimetapp, Vicks, Coricidin, Delsym, and others, including generic labels. Dextromethorphan has also found other uses in medicine to relieve primary psychological applications.The pain using dextromethorphan caused as cough suppressants, for temporary relief of cough due to small throat and bronchial irritation (how often accompanies the flu And the cold) and those who. Of inhaled irritants

Chondrus crispus carrageenan extract

Product: Chondrus crispus carrageenan extract
Description: A red / moss seaweed, abundant along the Irish coast and rich in oligosaccharides, which not only hydrate the skin, but also provides active ingredients deep into the epidermis.