Hugh-Leifson Medium

For the first time the method of (1 hour) rapid screening was developed by groups of Gram-negative bacteria through the OF test with glucose using mikrovolumetrischen techniques. The method is based on the use of hydrogen peroxide at a non-bactericidal concentration as a constituent of a liquid medium containing a buffer and a glucose indicator and is provided for the oxidation of glucose. Introduced for the investigation of the bacterial environment catalase ensures rapid saturation of the medium with oxygen and the completion of glucose oxidation in 10-60 minutes. A similar period is necessary in order to obtain a complete fermentation of the glucose in the same medium without hydrogen peroxide. It has been demonstrated that significant results have been obtained in the joint study of the OF-tests on the 502 strains belonging to the 21 genera and the bacteria which ferment Gram-negative nichtfermentierender, according to the process of hydrogen peroxide and the medium of Hugh-Leifson.