SHG-44 glioma cell-line

GOAL:
To study the effect of tamoxifen on voltage-sensitive sodium channels in SHG-44 glioma cells.
METHODS:
To record the Na currents in the SHG-44 cell line, a whole patch clamp of the cell was used and studied the effect of tamoxifen with different concentrations on this channel flow.
RESULTS:
This channel has been activated and deactivated quickly. Tamoxifen was able to greatly reduce the amplitude of the Na currents of the SHG-44 cell line. This block effect is dose-dependent and voltage dependent. When the holding potential was 0 mV, 8 micromoles / L tamoxifen was 69% block these fluxes. The half inhibitory concentration (IC 50) is 5.54 micromoles / liter.
CONCLUSION:
Tamoxifen could significantly block the voltage-dependent sodium channel in the SHG-44e malignant glioma cell line. It could be one of the mechanisms that inhibit tamoxifen glioma proliferation. Clamp technique was used to record Na currents in SHG-44 cell line and study the effect of tamoxifen with different concentrations on this channel flow.
RESULTS:
This channel has been activated and deactivated quickly. Tamoxifen was able to greatly reduce the amplitude of the Na currents of the SHG-44 cell line. This block effect is dose-dependent and voltage dependent. When the holding potential was 0 mV, 8 micromoles / L tamoxifen was 69% block these fluxes. The half inhibitory concentration (IC 50) is 5.54 micromoles / liter.
CONCLUSION:
Tamoxifen could significantly block the voltage-dependent sodium channel in the SHG-44e malignant glioma cell line. It could be one of the mechanisms that inhibit tamoxifen glioma proliferation.