Determination of myclobutanil 25% WG degradation dynamics in ginseng root

High-performance liquid chromatography coupled with a tandem mass spectrometry (HPLC / MS) method was developed 25% WG in ginseng root, stem, leaves and soil to determine kinetics Of degradation and the endreste of myclobutanil. The samples were extracted with purified acetonitrile with secondary amine primer (PSA) attached to the phase extraction cartridge by Kromasil Eternity-5-C18 (2.1 mm x 150 mm, 5 microm) of The column with a gradient of acetonitrile and 0.1% of formate separated in water as a mobile phase and analyzed at the multiple reaction monitoring (MRM) in the positive ion mode using the method of External standard. Mean coverage and relative standard deviations (DSR) of myclobutanil on the 0.01-0.20 mg / kg (-1) peak level amounted to 80.9% -90.7% and 5.54% -9, 29% and the limit of quantification (LQ) fraud 0.005 mg x kg (-1). The method with good, reproducible, high accuracy and low detection limit could meet the requirements of residue analysis of ginseng production. The half-lives of myclobutanil were 6.25 days to 9.94 days in root ginseng, stem, leaves and spray soil of 1,152 gx hm (-2). The endreste were among 0.060 1 mg x Kg (-1) in the root, 0.081 7 mg x kg (-1) in the boot, 0 to 0.102 0.006 2 mg x kg (-1) in the sheet and 0.037 6 mg x kg Of soil dosage range 576-1152 Gx hm (-2). It is recommended that myclobutanil MRLs can be assigned in dried ginseng at 0.10 mg kg (-1) x to be temporary, and the pre-harvest interval has been set at 35 days.