Cyclopamine decreased early embryonic development

Hedgehog path (Hh) has been studied in various life processes of the body of the animal and is proposed to be important for the development of several organs. The genes involved in the HH pathway were expressed in the ovary of mice, pigs and bovines. However, the function of the HH signaling pathway for oocyte maturation and early embryonic development is still controversial. We demonstrated the effect of Sonic hedgehog (Shh) and cyclopamine on the in vitro maturation of Mausozyten and the development of the embryo. The results showed that the presence of Shh resulted in cyclopamine oocyte maturation or similar to a control group. Shh not improved early embryonic development. However, the addition of depressed cyclopamine from early embryonic development. The shh, PTCH1, SMO and GLI1 mRNA was less detected in the bare oocytes. Expression levels of PTCH1 increased from the cleaved zygote to Blastozystenstufe. Smo or GLI1 were expressed in early embryonic separately at a higher level in the cell or four cell stages. Therefore Shh influenced the maturation of mouse oocytes and early embryonic development did not, however, cyclopamine resulted in inhibition of the early embryo development of the mouse. The effects of HH signaling in oocyte maturation and early embryonic development may be species specific.