What is Nectin-2

Nectin-2, a major protein component of adherent junctions (SJA), found between Sertoli cells and germ cells in the seminiferous epithelium. Recent studies have shown that the expression of the Nektin-2 gene is crucial for the maintenance of normal spermatogenesis in the testes, as male knockout mice lacking the Nektin-2 gene are sterile and have morphologically abnormal spermatozoa. However, the molecular mechanisms that control their basal transcription remain poorly understood. Has been demonstrated by the use of Sertoli and embryonic cell lines (TM4- and GC-2SPD (ts) cells) in transient transfection studies, that the minimal mouse Nektin-2 promoter between -316 and -211 nucleotides was The translation initiation site). In this region, two putative Sp1 motifs and a CRE, AP1 and AP2 motif were identified. Mutations studies showed that these two Sp1 motifs interact synergistically with the CRE motif, but not the AP1 and AP2 motifs at transcription of the Nektin-2 gene in both GC and TM4-2SPD (ts) - to regulate the cells. EMSA has been determined that an AP-1 consensus sequence can inhibit the formation of DNA-protein complexes with the CRE motif, suggesting an interaction between the AP-1 (c-Jun) and CREB transcription factor within Of the CRE suggests. Overexpression of the CREB and c-Jun protein, but not c-Fos, also significantly enhances promoter activity, suggesting that the CREB and c-Jun protein are the essential transcription factors that are involved in the Regulation of Neptin-2 transcription of the gene. Immunoprecipitation of chromatin showed that in vivo CREB, c-Jun and Sp1-family proteins were linked to the mouse Nektin-2 promoter. Tested tubule analysis confirmed that cyclic coincident expressions of CREB and nectin-2 with the case of adherens restructuring of junction between Sertoli cells and germ cells. Cross-talk between CREB, c-Jun and the Sp1-family protein is probably a great transcription machinery to drive Nektin-2 expression to drive in Sertoli cells.