Thioridazine

Thioridazine, an antipsychotic, it was reported that the induction of apoptosis in various cancer cell types, with a specificity of targeting cancer stem cells (TLC). Whether she coaxed the effects of cancer in gastric cancer has not been reported. In this study, we investigated thioridazine cell death capability in cell lines of gastric cancer AGS and NCI-N87 and found to induce her vivo inhibition of tumor capacity. Thioridazine cytotoxic effects induced on NCI-N87 cell AGS and a dose dependent manner and inhibited the formation of abilitiy colonies of NCI-N87 and AGS cells. Thioridazine nuclear fragmentation treatment is induced, increased the proportion of cells in G1 and sub-increased the percentage of Annexin V positive cells that. The occurrence of apoptosis Furthermore, thioridazine gastric cancer cells induced apoptosis in a caspase-dependent manner, as reversed by lower casapse-9 precursor caspase-8 and caspase-3, and by the ability of the inhibitor of caspase Z-VAD-FMK shown thioridazine cytotoxic effect. JC-1 staining was also found that gastric thioridazine apoptosis of cancer cells induced by the mitochondrial pathway. Further inhibited thioridazine NCI-N87 cell derived tumors pretreatment growth. The present study showed that the antipsychotic thioridazine gastric cancer-fighting ability in vitro and in vivo tests, suggesting thioridazine as a potential drug for the treatment of gastric cancer.