Farrerol Abstract

Farreyrol, (S) -2,3-dihydro-5,7-dihydroxy-2- (4-hydroxyphenyl) -6,8-dimethyl-4-benzopyrone isolated from rhododendron, was shown to have an anti potential -oxidative, but the molecular mechanism that underlying activity remains uncertain. Inducible expression of heme oxygenase-1 (HO-1), a potent antioxidant and cytoprotective enzyme is known to play an important role in cytoprotection in a variety of disease models. In this study, we evaluated playing against oxidative damage of the antioxidant potential farrerol and studied its antioxidant mechanism in RAW 264.7 cells. The molecular mechanism of the cytoprotective function of farrerol underlying determined by analysis of intracellular signaling pathways, activation of transcription and the inhibitory effect of HO-1 on the production of free radicals. farrerol antioxidant enzymes induced by the expression of HO-1 mRNA expression of the protein and the nuclear translocation of NF-E2-2 factor related to macrophage-like RAW 264.7 cells. farrerol down the expression of the protein and the thiol Keap1 means regulates attenuated induces a reduction farrerol HO- 1 expression. Other Western blotting investigations and specific inhibitors of Akt used p38, JNK and ERK shown that Akt, p38 and ERK signal ~~ axis POS = TRUNC mediates HO-1 expression. Furthermore, tert-butyl hydroperoxide (t-BHP) induced oxidative damage has been improved by a treatment farrerol in a dose-dependent manner Akt, p38, ERK and HO-1 inhibitors (NPS) was repealed. It is therefore likely that farrerol inactivated KEAP-1 or activated Akt, ERK and p38 release of Nrf2 and subsequent Keap1 decreases the intracellular generation of reactive oxygen species on the induction of HO -1 expression. These results confirm the central role of HO-1 in the cytoprotective effect of facilitating farrerol.