What is Obacunone

Overexpression of the enzyme aromatase was CYP19 involved in the development of breast cancer carcinogenesis estrogen dependent. Obacunone, a natural compound that has been found in citrus demonstrated for a variety of biological activities, including anti-cancer and anti-inflammatory. In this study, we obacunone and obacunone glucoside (OG) isolated from lemon seeds, then cut these connections chromatographic techniques and characterized by HPLC, LC-MS and 2D NMR spectral analysis. To examine the anti-cancer mechanism and anti-aromatase limonoids tested their cytotoxic effect on the human breast cancer breast cells (MCF-7) and non-malignant (MCF-12F). MTT assay confirmed that obacunone was strong MCF-7 cell proliferation is inhibited, affecting the breast without non-malignant cells. Treatment with obacunone increased apoptosis by upregulating the expression of pro-apoptotic protein Bax and downregulation of anti-apoptotic Bcl-2 and G1 cell cycle arrest inducing protein. Further obacunone aromatase activity in a significantly inhibited the in vitro enzyme assay. Exposure of MCF-7 breast cancer cells for expression downregulated obacunone of inflammatory molecules, including the nuclear factor kappa B (NFkB) and cyclooxygenase-2 (COX-2). In addition, we found that the activation of p38 mitogen obacunone by activated protein kinase (MAPK) COX-2 and NF? B is inhibited. Finally, the recording level obacunone in MCF-7 cells, as measured by HPLC, and its structure was confirmed by LC-HRMS. This study showed that obacunone have the potential can prevent breast cancer estrogen-sensitive by inhibiting the enzyme aromatase and inflammatory pathways, and activation of apoptosis.