Spiraeoside

Mast cells are responsible for allergic reactions IgE mediated by the secretion of various inflammatory cytokines and mediators. Thus, pharmacological regulation of mast cell activation is an important goal in developing new anti-allergic drugs. In this study, we found that spiraeoside (SP) and inhibits the activation of allergic reactions in vivo mast cells. dose-dependently inhibited SP-induced degranulation by IgE-antigen (Ag) in the stimulation of RBL-2H3 mast cells without cytotoxic effects. At the molecular level SP reduced Ag-induced phosphorylation and subsequent activation of phospholipase C-γ2 (PLC-γ2). SP also inhibited tyrosine kinase phosphorylation spleen (Syk), linker for activation of T cells (LAT) and downstream MAPK ERK1 / 2, p38 and JNK optionally attenuation of expression of TNF-α and IL-4. Finally, we found that SP anaphylaxis clearly IgE-mediated passive cutaneous (PCA) in the inhibited mouse. Taken together, our results suggest that activation suppressed SP IgE-mediated mast cell and allergic reactions by inhibiting the signaling / MAPK PLC-γ2 Lyn-induced in mast cells.