We analyze the functional state of smooth intra-muscle cells (n = 14 couples for age and sex) and the expression of relaxin-2 and its receptor RXFP2 RXFP1 and in samples of varicose veins and saphenous vein sound (VSG) (n = 21 healthy GSV; n = 46 varicose GSV). Relaxin-2 and its contents were RXFP1 (n = 9 per group) and samples determined in tissue samples. The pharmacological tests were carried out in a perfusion chamber. Morphometric determine the smooth muscle compartment base size showed no significant difference in varicose GSV compared to healthy controls. Relaxin-2 and its receptors have been entered into the muscle layer, the endothelial cells in blood vessels and in the wall of the vein for expression. immunohistochemical expression of relaxin-2, and RXFP1 RXFP2 varicose GSV has been significantly decreased. Relaxin-2 and RXFP1 measured by ELISA and Western Blot (2 relaxin ELISA were healthy varicose GSV reduced against varicose GVS: 12.49 ± 0.66 pg / mg compared with 9.12 ± 3.39 pg / mg total protein, p = 0.01, t test student). Contractions venous samples were induced by cholinergic or adrenergic stimulation unwell relaxin-2.
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