CRISPR pigs produce human proteins

Human serum albumin is a protein present in the blood, it can be used to treat a range of diseases, including liver failure and traumatic shock. Past have tried in pigs produce human serum albumin, but due to unavoidable produce swine albumin, which makes the separation and purification of human serum albumin source of great challenge.
Chinese scientists Zhangpu Min and his research team, using the CRISPR / Cas9 gene editing system, the fertilized egg in the pig genome region used to produce pig albumin, insert a paragraph can be synthesized DNA fragments of human albumin. Embedded in the pig genome allele located downstream of pig albumin synthesis genes. The researchers believe that this could make pigs produce human serum albumin, a source at the same time, do not produce swine albumin, serum albumin and so humanized swine albumin mixing can be avoided.
The researchers implanted 300 genomes edited fertilized eggs to 10 sows in vivo, which carry the fertilized eggs are inserted into a human serum albumin alleles. 5 pigs successful pregnancy, birth to 16 piglets Alabama. The researchers found that 16 pigs from a fertilized egg albumin gene that carries a human source, and they can be detected in human blood serum albumin. However, human serum albumin piglets body is different. Further breeding experiments showed that by CRISPR / Cas9 genetic modification tool into the human gene can be passed to the offspring of these piglets.