HL-7702

titanium oxide / chitosan composite scaffolds were prepared by a freeze-drying technique. Composite scaffolds were very porous with an average pore size of 120-300 microns, and titanium dioxide (TiO (2)) powders were uniformly dispersed on the surface of the pore walls. The compressive strength of the composite scaffolds was significantly improved compared to that of pure chitosan scaffolds. weight / chitosan composite scaffold with 0.3 TiO (2) showed resistance to the maximum compression 159.7 +/- 21 kPa. Liver immortal cell line HL-7702 was used as seed cells on the scaffold, and after periods of cultivation different investigated adhesion and cell function. HL-7702 in adhering to the walls of the scaffold pores with spheroid shape after 1 day of culture cells, but aggregates of cells in the formed TiO (2) / chitosan composite scaffolds compared to scaffolds of pure chitosan. specific functions of the liver, the secretion of albumin and urea synthesis were detected using a spectrometric method. The results showed that the secretion and urea synthesis rate of albumin HL-7702 cells slightly reduced with the time of culture, and there was no significant difference between the composite scaffolds and chitosan scaffolds pure. In conclusion, the TiO (2) / chitosan composite scaffolds had improved compared to pure chitosan scaffolds mechanical strength and supports the setting and functional expression of hepatocytes, which means that their possible application in tissue engineering of the liver.